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KMID : 0613820040140030425
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Journal of Life Science
2004 Volume.14 No. 3 p.425 ~ p.428
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Molecular Authentication and Genetic Polymorphism of Korean Ginseng (Panax ginseng C. A. Meyer) by Inter-Simple Sequence Repeats (ISSRs) Markers
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Bang Kyung-Hwan
Lee Sung-Woo
Hyun Dong-Yun
Cho Joon-Hyeong
Cha Seon-Woo
Seong Nak-Sul
Huh Man-Kyu
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Abstract
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Molecular authentication and genetic polymorphism of Korean ginseng cultivars and accessions were investigated using ISSR (inter-simple sequence repeat amplification) markers. Five primers among 56 produced clear and reproducible DNA fragments among seven cultivars and accessions. A total of 43 bands ranging from 250 bp to 1,700 bp from five primers were scored. Average number of bands per primer was 8.6 and only nine bands were polymorphic across the six Panax ginseng from Korea. Especially Chunpoong cultivar exhibited the highest level of polymorphism, whereas other accessions did not showed almost any polymorphism. Consequently, these ISSR markers will be available to differentiate Chunpoong cultivar from other major Korean ginseng cultivars and accessions, such as Yunpoong, Hwangsukjong and Jakyungjong, at the DNA level.
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KEYWORD
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Panax ginseng, molecular authentication, genetic polymorphism, ISSR marker
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